ABSTRACT
The prevalence of diabetes is increasing around the world. Oxidative stress is one of the risk factors in diabetes. The purpose of this study is to examine the protective effects of the essential oil of Satureja khuzestanica on the level of serum lipids and atherogenic indices in the male rats with diabetes. This experimental study was conducted on thirty male rats randomly divided into three groups including: 1- control group, 2- rats with untreated diabetes 3- rats with treated diabetes [through orally intake of water containing 500 ppm of the essential oil of Satureja khuzestanica]. The second and third groups also developed diabetes through injection of alloxan tetrahydrate [120 mg/kg]. After eight weeks of treatment, their blood sample was taken, and then the levels of glucose, lipids and atherogenic indices of the serum were measured. The levels of glucose, triglyceride, cholesterol, low density lipoprotein [LDL], very low density lipoprotein [VLDL] and atherogenic indices of plasma in treated diabetic group were significantly decreased. The amount of high density lipoprotein [HDL] was significantly increased in the treated group. The results obtained from this study showed that the essential oil of Satureja khuzestanica has beneficial effects on the level of serum lipids and atherogenic indices of rats with type 1 diabetes
ABSTRACT
<p><b>OBJECTIVE</b>To assess various antioxidative activities of Satureja khozestanica essential oil (SKE) and its effect on oxidation of low density lipoprotein (LDL) induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde (MDA).</p><p><b>METHODS</b>The formation of conjugated dienes, lag time and MDA were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE. Also total antioxidant activity and free radical scavenging of SKE were determinated.</p><p><b>RESULTS</b>It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation. The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200 µg/mL. Total antioxidant capacity of SKE was (3.20±0.40) nmol of ascorbic acid equivalents/g SKE. The SKE showed remarkable scavenging activity on 2, 2-diphenyl-picrylhydrazyl, IC50 (5.30±0.11) ng/mL.</p><p><b>CONCLUSIONS</b>This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.</p>
Subject(s)
Humans , Male , Antioxidants , Chemistry , Pharmacology , Copper Sulfate , Chemistry , Lipoproteins, LDL , Chemistry , Metabolism , Malondialdehyde , Chemistry , Oils, Volatile , Chemistry , Pharmacology , Oxidation-Reduction , Satureja , ChemistryABSTRACT
Oxidation of low-density lipoprotein [LDL] has been strongly implicated in the pathogenesis of atherosclerosis. The use of some natural antioxidant and herbal medicine may lead to the inhibition of production of oxidized LDL and may decrease both the development and the progression of atherosclerosis. The aim of this study was to investigate the effects of Olive leaves ethanol extract [OLE] on LDL oxidation induced-CuSO[4] quantitatively in vitro. Low-density lipoprotein was incubated with CuSO[4] and the formation of conjugated dienes and thiobarbituric acid reactive substances [TBARS]. Inhibition of this Cu-induced oxidation was studied in the presence of vitamin E and various concentration of OLE. It was demonstrated that OLE reduced the formation of conjugated dienes and TBARS of LDL against oxidation in vitro [P<0.05]. The inhibitory effects of the OLE on LDL oxidation were dose-dependent at concentrations ranging from [2microg/ml] to [200microg/ml]. Moreover, we compared effects of OLE on LDL oxidation with vitamin E as positive control. This study showed that OLE is a source of potent antioxidants and prevented the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications